The growth inhibition effect of arsenic trioxide injection on A549 cell line and influence on survivin expression

Objective:We investigate the inhibition effect on lung cancer cells and measured the variation of survivin expression level after treated with arsenic trioxide in vitro. Our objective is to illuminate the possible mechanism of arsenic trioxide on lung cancer. Methods:Lung cancer cell line-A549 cells were cultured in vitro. Different doses of arsenic trioxide were co-cultured with A549 cells and the terminal concentrations were 0.5, 1.0, 2.0, 4.0μmol/L. Cell viability was counted by trpan-blue staining and cell growth inhibition rate was calculated. After centrifuged for 5 min at the condition of 1 000rpm, the sediments were collected for western blot experiment according to the molecular cloning experiment guideline. In this section, the correlation of survivin expression levels inhibited by arsenic trioxide with survival rate of A459 cells was analyzed. Results:A549 cell growth was markedly inhibited by arsenic trioxide as concentration-and time-dependent manner. The level of survivin expression was sown-regulated by arsenic trioxide and also as concentration-and time-dependent manner. The survivin protein level was negatively correlated with the tumor cell survival rate. Conclusion:A549 cell growth is markedly inhibited by arsenic trioxide in vitro. Arsenic trioxide is an effective agent which can inhibit lung cancer cells through inhibiting survivin expression.