Analysis of thermophilic bacterial communities structure in high temperature composting of kitchen waste.

Using base on the polymerase chain reaction (PCR) of 16S rDNA-denaturing gradient gel electrophoresis (DGGE) technique and phylogenetic analysis, the thermophilic bacterial communities structure in high temperature composting of kitchen waste was studied. The composting high-temperature stage (50℃ ) continued 7 days, in this period, sampling stack body and collecting microbial total DNA of the object, 16S rDNA fragments were amplified using universal bacteria primers (GC-341F/907R). The DGGE analysis was carried out on amplification of 16S rDNA. The DGGE twig belt similarity Cs value analysis and cut glue survey order, using order arrange data to carry out same source property analysis, and system phylogenetic tree was established. The analysis results of DGGE and similarity Cs value showed that the composting high temperature stage had more rich bacteria diversity and existed obvious superior community structure change.The analysis results of cut survey order and phylogenetic showed that large part of high temperature stage superior community order arrange possessed nearer blood relation with Bacillus and Clostridium.