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2008•Journal of Hefei UniversityRequires access

A Research on Microbial Community in Aerobic Composting by 16S rDNA

Jie Jin

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Abstract

The species diversity of bacterial communities in aerobic composting is investigated by coupling culture-dependent methods to PCR-DGGE(denaturing gradient gel electrophoresis) targeting 16S rRNA gene(rDNA).The results of plate counting indicate that population of bacteria composting process is higher-lower-lower trend,DGGE patterns during different periods show the presence of different populations,a few dominant species existed throughout the composting process.PCR-DGGE techniques provide useful molecular microbial ecology in assessing the changes in microbial community structure of composting.

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What this paper is about

The species diversity of bacterial communities in aerobic composting is investigated by coupling culture-dependent methods to PCR-DGGE(denaturing gradient gel electrophoresis) targeting 16S rRNA gene(rDNA).The results of plate counting indicate that population of bacteria composting process is higher-lower-lower trend,DGGE patterns during different periods show the presence of different populations,a few dominant species existed throughout the composting process.PCR-DGGE techniques provide useful molecular microbial ecology in assessing the changes in microbial community structure of composting.

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Available abstract

The species diversity of bacterial communities in aerobic composting is investigated by coupling culture-dependent methods to PCR-DGGE(denaturing gradient gel electrophoresis) targeting 16S rRNA gene(rDNA).The results of plate counting indicate that population of bacteria composting process is higher-lower-lower trend,DGGE patterns during different periods show the presence of different populations,a few dominant species existed throughout the composting process.PCR-DGGE techniques provide useful molecular microbial ecology in assessing the changes in microbial community structure of composting.

Key concepts: Temperature gradient gel electrophoresis, 16S ribosomal RNA, Microbial population biology, Biology, Bacteria, Microbial ecology, Molecular ecology, Population

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