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2015•Tianran chanwu yanjiu yu kaifaRequires access

Optimization of Extraction Conditions of Polysaccharide from Potentilla anserina by Response Surface Methodology

Gao Dan-da

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Abstract

The extraction technology of the water-soluble polysaccharide from Potentilla anserina was studied in this paper. The influences of liquid-solid ratio( V / W),extraction time and temperature on the extraction yield were investigated. On the basis of single factor tests,a Box-Behnken design( BBD) was used to study the influence of the 3 independent variables on the extraction yield. The results showed that the optimal extraction parameters were as follows: the liqudsolid ratio was 20. 25∶ 1,extraction time was 4. 37 h and extraction temperature was 82 ℃. The extraction yield of polysaccharide was 24. 53 ± 0. 24%.

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What this paper is about

The extraction technology of the water-soluble polysaccharide from Potentilla anserina was studied in this paper. The influences of liquid-solid ratio( V / W),extraction time and temperature on the extraction yield were investigated. On the basis of single factor tests,a Box-Behnken design( BBD) was used to study the influence of the 3 independent variables on the extraction yield. The results showed that the optimal extraction parameters were as follows: the liqudsolid ratio was 20. 25∶ 1,extraction time was 4. 37 h and extraction temperature was 82 ℃. The extraction yield of polysaccharide was 24. 53 ± 0. 24%.

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Available abstract

The extraction technology of the water-soluble polysaccharide from Potentilla anserina was studied in this paper. The influences of liquid-solid ratio( V / W),extraction time and temperature on the extraction yield were investigated. On the basis of single factor tests,a Box-Behnken design( BBD) was used to study the influence of the 3 independent variables on the extraction yield. The results showed that the optimal extraction parameters were as follows: the liqudsolid ratio was 20. 25∶ 1,extraction time was 4. 37 h and extraction temperature was 82 ℃. The extraction yield of polysaccharide was 24. 53 ± 0. 24%.

Key concepts: Extraction (chemistry), Yield (engineering), Polysaccharide, Response surface methodology, Chromatography, Potentilla, Chemistry, Botany

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