Optimization of the Extraction Process of Polysaccharide from Gloeostereum incarnatum by Response Surface Methodology

The objective of this research was to optimize the extraction conditions of Gloeostereum incarnatum by using water in order to enhance the extraction rate of polysaccharide. Based on single factor and Box-Behnken experiment design, response surface methodology was used to optimize the conditions of extraction technique of polysaccharide from Gloeostereum incarnatum. In order to improve the extraction ratio, extraction temperature, extraction time, solid to liquid ratio and extraction cycle were optimized by response surface methodology. The results showed that the optimal hydrolysis conditions of the polysaccharide ratio were as followed: extraction temperature was 84.925 ℃; extraction time was 3.477 h; solid to liquid ratio was 1 ∶ 50(g/m L)and the extraction cycle was 4. Under the optimal conditions the theoretical extraction ratio of polysaccharide was 9.596 %. Among the series of conditions, the importance effects on extraction ratio were ranged as extraction time, extraction temperature, solid to liquid ratio and extraction cycles.