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2011•Zhongliu fangzhi yanjiuOpen access

Effect of siRNA Targeted against Bcl-x_L on Cell Proliferation and Apoptosis of Prostate Cancer Cells

Zhitu Zhu

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Abstract

Objective To investigate the effect of siRNA targeted against Bcl-xL on cell proliferation and apoptosis of PC-3 prostate cancer cells.Methods A siRNA plasmid expression vector against Bcl-xL was constructed and transfected into PC-3 cells.The changes of Bcl-xL mRNA and protein expression were detected by semi-quantitive RT-PCR and Western blot respectively.Cell proliferation was detected by MTT assay.Cell apoptosis was evaluated by flow cytometry.Results The sequence-specific siRNA effectively suppressed Bcl-xL expressing at both mRNA and protein levels.Bcl-xL expression significantly inhibited the proliferation(P0.001)and induced apoptosis of PC-3 cells(P0.001) respectively.Conclusion siRNA targeted against Bcl-xL could effectively suppress PC-3 cell proliferation and significantly induce PC-3 cell apoptosis.

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Objective To investigate the effect of siRNA targeted against Bcl-xL on cell proliferation and apoptosis of PC-3 prostate cancer cells.Methods A siRNA plasmid expression vector against Bcl-xL was constructed and transfected into PC-3 cells.The changes of Bcl-xL mRNA and protein expression were detected by semi-quantitive RT-PCR and Western blot respectively.Cell proliferation was detected by MTT assay.Cell apoptosis was evaluated by flow cytometry.Results The sequence-specific siRNA effectively suppressed Bcl-xL expressing at both mRNA and protein levels.Bcl-xL expression significantly inhibited the proliferation(P0.001)and induced apoptosis of PC-3 cells(P0.001) respectively.Conclusion siRNA targeted against Bcl-xL could effectively suppress PC-3 cell proliferation and significantly induce PC-3 cell apoptosis.

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Available abstract

Objective To investigate the effect of siRNA targeted against Bcl-xL on cell proliferation and apoptosis of PC-3 prostate cancer cells.Methods A siRNA plasmid expression vector against Bcl-xL was constructed and transfected into PC-3 cells.The changes of Bcl-xL mRNA and protein expression were detected by semi-quantitive RT-PCR and Western blot respectively.Cell proliferation was detected by MTT assay.Cell apoptosis was evaluated by flow cytometry.Results The sequence-specific siRNA effectively suppressed Bcl-xL expressing at both mRNA and protein levels.Bcl-xL expression significantly inhibited the proliferation(P0.001)and induced apoptosis of PC-3 cells(P0.001) respectively.Conclusion siRNA targeted against Bcl-xL could effectively suppress PC-3 cell proliferation and significantly induce PC-3 cell apoptosis.

Key concepts: Apoptosis, Transfection, Cell growth, Flow cytometry, Molecular biology, Western blot, Cell, Bcl-xL

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Effect of siRNA Targeted against Bcl-x_L on Cell Proliferation and Apoptosis of Prostate Cancer Cells — Research Paper | ScholarLens