Change of Albumin Fraction in Barley Seeds during Germination

The change in content of albumin fraction during germination was studied in this paper.Four species barleys,Hordeum vulgare L.Kenpi.No.8,Hordeum vulgare L.Ganpi.No.4,Hordeum vulgare L.Harringto and Hordeum vulgare L.Esterel were used to detect the change in content of albumin fraction during germination with the method of Coomassie brilliant blue G-250,then run SDS-PAGE.Experimental results are showed as follows,the content of albumin is more times from 1.4 to 2.3 in malts germinated six days than in barley seeds.SDS-PAGE analysis showed that albumin in barley seeds and albumin subunits exists in the district between 10 to 66 kilodaltons.More spectral bands appeared in molecular weight of 25~45 kilodaltons separation than others during germination.The content of albumin fraction in different barley seeds demonstrates more obvious regularity during germination,it was deficient in HMW protein components,but rich in MMW and LMW protein subunits