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2008•Journal of Nuclear Agricultural SciencesRequires access

THE OVERCOMING PRE-FERTILIZATION BARRIERS OF LILY VIA ASSISTING POLLINATION OF IRRADIATED POLLEN

Lio Jun, Kezhong Zhang, Yuehui Jia, Qixiang Zhang, Ma Li

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Abstract

The effect of irradiated pollen was studied on overcoming incompatibility and incongruity in LA lily cultivar'Dazzle'(♀)×Lilium davidii.After 3～7 days of pollination,the pollen-tube growth in pistils was observed with fluorescence microscope.The results showed as follows: for normal pollination,pollen tubes stopped growthing in the styles when they strengthened to some length,forming callose at the top of them.For the pollination of mentor pollen and pioneer pollen,pollen tubes entered the ovary,and approached or might penetrated into the ovules.This indicated that the assisting pollination methods with irradiated pollen were able to overcome the pre-fertilization barriers of 'Dazzle'(♀)×Lilium davidii partly.

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What this paper is about

The effect of irradiated pollen was studied on overcoming incompatibility and incongruity in LA lily cultivar'Dazzle'(♀)×Lilium davidii.After 3～7 days of pollination,the pollen-tube growth in pistils was observed with fluorescence microscope.The results showed as follows: for normal pollination,pollen tubes stopped growthing in the styles when they strengthened to some length,forming callose at the top of them.For the pollination of mentor pollen and pioneer pollen,pollen tubes entered the ovary,and approached or might penetrated into the ovules.This indicated that the assisting pollination methods with irradiated pollen were able to overcome the pre-fertilization barriers of 'Dazzle'(♀)×Lilium davidii partly.

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Available abstract

The effect of irradiated pollen was studied on overcoming incompatibility and incongruity in LA lily cultivar'Dazzle'(♀)×Lilium davidii.After 3～7 days of pollination,the pollen-tube growth in pistils was observed with fluorescence microscope.The results showed as follows: for normal pollination,pollen tubes stopped growthing in the styles when they strengthened to some length,forming callose at the top of them.For the pollination of mentor pollen and pioneer pollen,pollen tubes entered the ovary,and approached or might penetrated into the ovules.This indicated that the assisting pollination methods with irradiated pollen were able to overcome the pre-fertilization barriers of 'Dazzle'(♀)×Lilium davidii partly.

Key concepts: Pollen tube, Pollen, Callose, Gynoecium, Pollination, Lilium, Ovule, Biology

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