2004•Zhongguo shouyi ke-jiRequires access

Development of indirect ELISA with recombinant protein as antigen

Qin Rui-ling, Xichen Zhang, Jianhua Li, XU Zhan-yun, Jigang Yin, Yang Ju

Abstract

The indirect ELISA for detection of serum antibodies to Eimeria tenella was established with the recombinant fusion protein expressed in E.coli as antigen and mice anti-chicken IgG HRP conjugate as the second antibody.The results showed that coating antigen was 1 μg per well for λMzp5-7 recombinant protein, and blocked with 100 mL/L serum of foetus bovine. The assay was characterized by simplicity,rapidity and economy.

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Available abstract

Key concepts: Recombinant DNA, Antigen, Biology, Antibody, Fusion protein, Molecular biology, Conjugate, Virology