Staining methods for sections of epon-embedded tissues for light microscopy
C. Roland Leeson, Thomas S. Leeson
Abstract
C. Roland Leeson, Thomas S. Leeson
Abstract
Sections 0.5–2 μ thick are mounted on clean glass slides and allowed to dry. A number of staining procedures are described. After the sections are stained, permanent preparations are made by mounting them in a synthetic resin. The methods result in sections which are suitable for routine light microscopy and for comparison with adjacent electron microscopic sections.
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Sections 0.5–2 μ thick are mounted on clean glass slides and allowed to dry. A number of staining procedures are described. After the sections are stained, permanent preparations are made by mounting them in a synthetic resin. The methods result in sections which are suitable for routine light microscopy and for comparison with adjacent electron microscopic sections.
Key concepts: Staining, Microscopy, Biology, Electron microscope, Microtome, Optical microscope, Anatomy, Pathology