Temporary Expression of Human Lysozyme cDNA in COS-1 Cells and Murine Mammary Gland

Objective To study the characteristics of expression of self-cloned 1.5 kb hLYZ cDNA.Methods Subclone the 1.5 kb hLYZ cDNA into an eukaryotic expression vector pcDNA3 and mammary gland expression vector p205C3.Transfect COS-1 cells with recombinant plasmid pcDNA3LYZ and detect the expression of hLYZ cDNA by IFA.Inject the mice during lactating period with recombinant plasmid p205C3LYZ and detect the expression of hLYZ cDNA in vivo of mice by micrococcal lysis assay and dot blot.Results hLYZ cDNA was correctly expressed in the transfected COS-1 cells.The mean expression level of hLYZ in milk of mice was 87 μg/ml.The expression of target protein was tissue-specific.Conclusion The self-cloned hLYZ cDNA may be used for the preparation and development of recombinant hLYZ.The recombinant plasmid p205C3LYZ may be used for the preparation of animal mammary gland bioreactors.