2010•Northern HorticultureRequires access
Tissue Culture of Robinia. pseudoacaciaf. unifolia
Jinmao Wang
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Abstract
Stems with axillaries bud of Robinia.pseudoacaciaf.unifolia were used as explants,this paper probed into the effects of 6-BA,NAA,IBA on proliferation and plantlet regeneration of Robinia.pseudoacaciaf.unifolia.The results showed that the medium suitable for bud germination was MS+6-BA 1.0 mg/L;The optimum multiplication culture medium was MS+BA 1.0 mg/L+NAA 0.2 mg/L;The rooting culture medium was that the clones were cultured 7 d in 1/2MS+IBA 50 mg/L,then were cultured 10 d in 1/2MS0 medium.In this system,the rate of induction of 95.0% the highest propagation coefficient was 5.72 and the rooting rate was 83.3% and the survived plantlets rate of transplant was 100%.