2011•Anhui nongye kexueRequires access
Research on the Selection of High-yielding Chitosanase Strain and Its Mutagenesis Breeding
Haibo Wang
Open publisher page 0 citations
Abstract
[Objective] The research on the strain producing the chitosan with high activity,mutated with ultraviolet and diethyl sulfate(DES) was conducted.[Method] One strain of high-yielding chitosanase was isolated in the selective medium with the chitosan as sole carbon source and then was identified.Its ability in enzyme producing was improved through the treatment of UV and DES mutation.[Result] The preliminary identification indicated that the strain Y-4 producing the chitosanase might be one of Aspergillus fumigatus.The activity of two mutants,which was from the treatment of UV and DES,was 8.92 and 8.12 U/ml,respectively,which was 3.0 and 2.7 times of the strain Y-4(3.00 U/ml),respectively.Two mutant strains had been stable in enzyme production after serial of 10 generations.[Conclusion] The effect of the ultraviolet radiation mutagenesis was better than that of DES.