Ionization of Calcium in Cow Bones

In order to study calcium in cow bones,extraction of ionized calcium was conducted by mechanical fragmentation combined with electrolysis technology in the mixed solvent of citric acid and lactic acid.Through the comparison of single factor experiments and orthogonal test,the optimal extraction parameters were 40-60 ℃ of water-bath temperature,50-90 r/min of stirring speed,20 V of voltage,3 h of treatment,1:2 of bone and mixed acid ratio with equal amount of citric acid and lactic acid,2% bone powder concentration.The final calcium extraction rate was 96.37%.No calcium in bone residues was determined after electrolysis,which suggesting the ionization of total calcium.This technology exhibited advantages of high dissolution rate,excellent solubility and fast absorption capability in gastrointestinal tract for ionized calcium,and also provided a practical and reliable method for comprehensive utilization of animal bones.