Extraction Methods of PCR-detectable Escherichia coli Genomic DNA in Milk

In order to obtain an appropriate sample for PCR detection, four extraction methods including saturated phenol method, CTAB method, kit method and solvent pyrolysis method for E. coli genomic DNA in milk samples were compared. Through evaluating the purity, quantitative analysis and PCR analysis of genomic DNA extracted by four methods, an effective and fast solvent-thermal pyrolysis method was suitable for the extraction of E. coli genomic DNA in milk. The genomic DNA extracted by this method used as the template is suitable for PCR amplification of E. coli DNA, which can be used for the detection of E. coli in milk.