Effects of Rxa on L02 cell proliferation and the expression of Ki 67, Cyclin D1, PCNA protein

Objective To investigate the effect of Rxa, a kind of extract of RSM, on the proliferation rate in cultured human hepatocytes (L02 cells). Methods L02 cells were divided into two groups: non treatment group (L group) and Rxa treated group (La group, Rxa 2 mmol/L). Indices of G0 G1 phase cells, S phase cells and G2 M phase cells were measured by using flow cytometry at 0, 2, 4, 8 and 12 h, respectively, and the expression of Ki 67, Cyclin D1, PCNA protein were simultaneously detected by using immunohistochemical method. Results Index of hepatocytes in each phase reached the peak more quickly in Rxa treated group than in non treatment group, and the Ki 67 expression was also higher than that one. Cyclin D1 and PCNA expressed earlier in the hepatocytes treated with Rxa ( P 0.01), which expressed sooner than in the hepatocytes not treated with Rxa about 2～4 h. Conclusion Rxa may be play an important role in the hepatic proliferation, which may be contributed to the triggering expression of Ki 67, Cyclin D1 and PCNA, by which the cell cycle transport was speeded up.\;