Screening of a high alcohol tolerant yeast and study on its fermentation performance

To isolate high alcohol tolerant yeast,enrichment culture was used to separate a yeast named A2 from grain stillage of Chinese liquor,which could tolerant the concentration of alcohol at 18%vol.The optimum growth temperature and pH of A2 were 32℃ and 4.5,respectively.The optimum fermentation conditions of A2 were obtained by orthogonal experiments as follows: glucose 20%(w/v),fermentation 34℃ and initial pH 4.5.After 72h of fermentation,the yield of alcohol reached 9.5%vol.