Photoprotection of Skin Fibroblasts from Ultraviolet Radiation by Notoginsenoside R1

Objective To observe the photoprotection of notoginsenoside R1 on fibroblasts after UVB and UVA irradiation and to explore the relative molecular mechanism.Methods Fibroblasts were obtained from human skin.The cells were irradiated with different dosages of UV and treated with notoginsenoside R1.The damage of fibroblasts was observed with inverted phase contrast microscope.Cellular proliferation activity was detected by MTT method.The hydroxyproline amount in the culture supernatant was measured by colorimetric method,and the level of matrix metalloproteinase-1(MMP-1) secreted by fibroblasts was measured with ELISA kits.Results The irradiation damage degree of the cells was dependent with the irradiated dose.The number and morphology of the cells remained normal when they were pretreated with notoginsenoside R1.The activity of proliferation of fibroblasts irradiated by UV was increased and MMP-1 protein secretion level was suppressed when the dose of notoginsenoside R1 was 5 μg· mL-1 and 20 μg· mL-1.Conclusion UVA and UVB irradiation can induce obvious damage to fibroblasts of human skin.Notoginsenoside R1 protects the damage of fibroblasts from UV irradiation.