2006•Biotechnology(Faisalabad)Requires access

Compound Mutagenesis of HNO_2 and UV of Protoplast on Screening of Enzyme-producing Strain

Xiao Huai-qiu

Abstract

Objective:A stable,high-yield neutral proteinase-producing strain can be screened.Methods:Compound mutagenesis of UV and HNO_2 on protoplast from Bacillus subtiIis UV_(11) was launched and protoplast was prepared at its optimal formation and regeneration conditions.Re- suits:A mutant strain Bacillus subtilis UN_(19) was obtained and its neutral proteinase-producing capacity was improved from 378.97U/ml to 3965.84U/ml.Condusion:The method of compound mutagenesis of HNO_2 and UV of protoplast for screening of high producing strain was con- firmed as a good way.

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Key concepts: Protoplast, Bacillus subtilis, Mutagenesis, Strain (injury), Enzyme, Proteinase K, Mutant, Yield (engineering)