2010•Food Science and Technology InternationalRequires access
Screening of high-yield β-Glucanase producing mutants by protoplast UV mutagenesis
Zhiqun Liang
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Abstract
The protoplasts of Aspergillus fumigatus was prepared by dissolving it with mixed-enzyme system.After the protoplasts were induced by ultraviolet ray and LiCl,a high-yield strain UV-26 was obtained by first screening of flat plate and second screening of shake flask.The enzymatic ability of the strain was increased from the initial 1.1 U/mL to 3.4 U/mL.And it can descend stably after six generation.