2012Shandi Nongye Shengwu xuebaoRequires access

Genetic Diversity of 101 Maize Germplasms from Guizhou Province as Revealed by SSR Markers

Bai Guang-xiao

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Abstract

The genetic diversity of 101 maize germplasms originated from Guizhou Province was analyzed by the SSR molecular markers generated from 23 pairs of stable SSR primers,which screened from 220 pairs.A total of 252 alleles were detected,and the average alleles per SSR loci were 11 with a range from 7 to 21.The Polymorphic Information Content(PIC) for the SSR loci varied from 0.753 to 0.945 with an average of 0.857.Genetic dissimilarity coefficients ranged from 0.722 to 0.905 with an average of 0.812.Taking 0.80 of genetic dissimilarity coefficient as thresheld,the 101 maize germplasms might be divided into five groups by UPGMA method based on SSR markers.The far genetic distance was investigated between the 5th group and other four ones.This separated group was Dahuangzao from Ceheng County.Therefore,the genetic relationships among these maize germplasms by SSR marker may provide a theoretical basis for further breeding.

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The genetic diversity of 101 maize germplasms originated from Guizhou Province was analyzed by the SSR molecular markers generated from 23 pairs of stable SSR primers,which screened from 220 pairs.A total of 252 alleles were detected,and the average alleles per SSR loci were 11 with a range from 7 to 21.The Polymorphic Information Content(PIC) for the SSR loci varied from 0.753 to 0.945 with an average of 0.857.Genetic dissimilarity coefficients ranged from 0.722 to 0.905 with an average of 0.812.Taking 0.80 of genetic dissimilarity coefficient as thresheld,the 101 maize germplasms might be divided into five groups by UPGMA method based on SSR markers.The far genetic distance was investigated between the 5th group and other four ones.This separated group was Dahuangzao from Ceheng County.Therefore,the genetic relationships among these maize germplasms by SSR marker may provide a theoretical basis for further breeding.

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Available abstract

The genetic diversity of 101 maize germplasms originated from Guizhou Province was analyzed by the SSR molecular markers generated from 23 pairs of stable SSR primers,which screened from 220 pairs.A total of 252 alleles were detected,and the average alleles per SSR loci were 11 with a range from 7 to 21.The Polymorphic Information Content(PIC) for the SSR loci varied from 0.753 to 0.945 with an average of 0.857.Genetic dissimilarity coefficients ranged from 0.722 to 0.905 with an average of 0.812.Taking 0.80 of genetic dissimilarity coefficient as thresheld,the 101 maize germplasms might be divided into five groups by UPGMA method based on SSR markers.The far genetic distance was investigated between the 5th group and other four ones.This separated group was Dahuangzao from Ceheng County.Therefore,the genetic relationships among these maize germplasms by SSR marker may provide a theoretical basis for further breeding.

Key concepts: UPGMA, Genetic diversity, Biology, Allele, Germplasm, Genetic distance, Microsatellite, Molecular marker

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