Extraction and Purification of Microcystins-LR,RR
Xiaogang Feng
Abstract
Xiaogang Feng
Abstract
Some new methods were established to isolate and purify Microcystins from natural cyanobacterial bloom,by the main steps of extraction with aqueous methanol and solid phase extraction.The optimized method was evaluated with different solvents applied to assess the efficiency of microcystins extraction.60% methanol had the highest efficiency,and the massive of phycobiliprotein was removed by adjusting the pH of solvents to the isoelectric point.This result provided a solid basis for obtaining pure microcystins efficiently.
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Some new methods were established to isolate and purify Microcystins from natural cyanobacterial bloom,by the main steps of extraction with aqueous methanol and solid phase extraction.The optimized method was evaluated with different solvents applied to assess the efficiency of microcystins extraction.60% methanol had the highest efficiency,and the massive of phycobiliprotein was removed by adjusting the pH of solvents to the isoelectric point.This result provided a solid basis for obtaining pure microcystins efficiently.
Key concepts: Chromatography, Extraction (chemistry), Chemistry, Solid phase extraction, Methanol, Aqueous solution, Isoelectric point, Organic chemistry