2011•Advanced materials researchOpen access

Study on the Extraction and Purification of Microcystins (MC-LR)

Wen Yi Zhang, Ning Han, Li Rong Yao, Xiao Lan Qiu, Xiao Liang Chen

Abstract

The MC-LR from the the blue-green algae of Taihu Lake was extracted, at the same time, a set of microcystins extraction method with methanol as extraction solvent and purification method with C18-SPE as purification workstations were established. The extraction solvent concentration, extraction time, extraction solvent amount, leacheate concentration and eluent concentration were used to research the extraction efficiency of MC-LR. Finally, 80% methanol was used to wash microcytins to make MC-LR high purity and the purity was over 85%. This research presented a method of low cost and high efficiency. It provided the foundation for the further research of microcytins.

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Available abstract

Key concepts: Extraction (chemistry), Chromatography, Solvent, Methanol, Chemistry, Organic chemistry