Changes of calcitonin gene-related peptide in irritable bowel syndrome

To explore the serum change of calcitonin gene-related peptide(CGRP) in irritable bowel syndrome(IBS).Methods A total of 30 male Wistar rats were randomly divided into model group,control group and normal group.The rat model of IBS was established by pouring 0.85 mL trinitrobenzenesulfonic acid(TNBS) 8 cm deep into the colon.In control group,same volume of normal saline instead TNBS.No treatment was carried on nomal group. They were executed 30 days later.Before and after the model duplication,an abdominal withdrawal reflex(AWR) scoring system was used to evaluate improvements of the threshold of visceral sense.Then an CGRP ELISA kit was used to measure the concentration of serum CGRP.Results The threshold of visceral sense was significantly lower in model group than that in control group and normal group,and significantly lower than before pouring TNBS(P0.05).The serum concentration of CGRP in model group was significantly higher than that in control group and nomal group(P0.05).The concentration of CGRP was higher in cotrol group than that in normal group,but there was no difference(P0.05).Conclusion The concentration of CGRP rose in IBS rat model,it may play a role in the generation of IBS.