Experimental Study on Apoptosis Induced by Ethanol Extracts of Solanum Lyratum Thunb in Human Lung Cancer SPC-A-1 Cells

OBJECTIVE:To explore the effects of ethanol extracts of Solanum lyratum Thunb(ST) on induction of apoptosis and the expression of apoptosis associated genes fas and caspase-3 in human lung cancer SPC-A-1 cells.METHODS:Cultured human lung cancer SPC-A-1 cells were randomly divided into the control group,ethanol extracts of ST treated groups(2.5、5、10 mg·L-1)and the positive control group(cisplatin).After treatment with drug for 48 h,the proliferation inhibitory rate was evaluated by MTT assay,induction of cell apoptosis rate was determined by TUNEL method,the expression of fas and caspase-3 mRNA was detected by semi-quantitive RT-PCR.RESULTS:Compared with control group,the inhibitory rate was increased obviously(P0.001),the apoptotic rate was increased markedly(P0.05 or P 0.01),The expression of fas and caspase-3 mRNA was increased significantly(P0.05 or P 0.01) in ST groups.CONCLUSION:ST may induce apoptosis by up-regulating expression of fas and caspase-3 genes,and inhibit the development of SPC-A-1 cells.