Detection of Extended-spectrum Beta-lactamases and Analysis of Antibiotic Resistance in Clinical Isolates of Klebsiella

OBJECTIVE To determine the antibiotic resistance and the productive rate of extended-spectrum-beta-lactamases (ESBLs) in the clinical isolates of Klebsiella. METHODS To detect the ESBLs and antibiotic resistance of Klebsiella, 207 strains of Klebsiella were isolated from the patients admitted to the Guangzhou First Municipal Hospital during the years 2000 and 2001, then antibiotic susceptibility test was peformed by Kirby-Baucer disk diffusion method, ESBLs production was performed by the standard disk diffusion method. RESULTS ESBLs productive rate increased significantly from 28% to 39% during the years 2000 and 2001. ESBLs-producing strains had higher resistance rate than that of non-ESBLs-producing ones. ESBLs-producing strains were only sensitive to the combination of third-generation cephalosporins with beta-lactamase inhibitor, fourth-generation cephalosporins and imipenem. Imipenem had the most effective antibiotic activity among them. CONCLUSIONS ESBLs production of Klebsiella had significantly increased. ESBLs-producing strains were the most sensitive to imipenem. Measures should be taken to control the spread of ESBLs-producing Klebsiella.