Effect of Cyclosporin A on inflammatory response of cerebral ischemia-reperfusion-injury in rats

Objective: To explore the protective effects of Cyclosporin A on the cerebral ischemia-reperfusion-injury in rats. Methods: 108 wistar rats were divided into 3 groups, which were sham operation group,saline control groups and cyclosporin A treatment group. The focal cerebral ischemia-reperfusion-injury model was made by suture-occluded method. The score of behavior obstacles in rats was rated. TTC staining was used to calculate the cerebra infarct volume. After 2 h MCA occlusion following 22 and 70 h reperfusion, bath the activity of myeloperoxidease (MPO) and the content of interleukin-1β (IL-1β) were measured in ischemia cerebra area. Results: Compared between the cyclosporin A group and the control group in scheduled time, the score of behavior obstacles in the cyclosporin A group was better than that of the control group (P 0.05); the infarct volumes of cerebra was markedly less than that of the control group (P 0.05); in ischemia cerebral zone bath the activity of MPO and the content of IL-1β in cyclosporin A group were significantly lower than those in the control group (P 0.01). Compared to other two groups, the sham operation group have no obvious abnormal change in each observational item. Conclusion: Inflammatory response play important role in cerebral ischemic reperfusion injury in rats. Cyclosporin A can reduce the content of IL-1β and inhibit the leukocyte infiltration, which produce protective effect on cerebral ischemic reperfusion injury in rats after focal cerebral ischemia.