2010•Chinese Journal of Ethnomedicine and EthnopharmacyRequires access

Determination of Quercetin content in Dracoephalum mololariea L by RP-HPLC

Liang Ge

Abstract

Objective:To establish a method for determination of the content of quercetin in Dracoephalum mololariea L.Metheds:Kromasil C18(4.6×150mm,5um)were used,Acetonitrile-5% Formic acid(31:69)was served as mobile phase,and flow rate was 1.0ml·min-1,wavelength for determine was 280 nm.Results:The calibration curves were linear in the range of 0.02mg·mL-1~0.10mg·mL-1(r=0.9990),The average recovery rates with the RSD were 97.2 %(1.2%),respectively.Conclusion:The method is accurate,and suitable for the determination of the content of quercetin in Dracoephalum mololariea L.The method can be adopted for quality control of Dracoephalum mololariea L.

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Key concepts: Quercetin, Chromatography, Formic acid, Chemistry, Content determination, Calibration curve, High-performance liquid chromatography, Linear range