Improvement of the thiocyanate in milk by ion chromatography method

Objective To enhance the sample pretreament method and chromatographic condition for improvement of the thiocyanate in milk by ion chromatography. Methods The proteins in milk were precipitated by heating sodium hydroxide solution.Thiocyanate was separated with AS14 anion exchange column, eluted with 3.5 mmol/L Na2CO3-1.0 mmol/L Na HCO3, and quantified with conductivity detector. Results The linearity range of this method was from 0.1 mg/L-2.00 mg/L. The regression equation was y=1.314×10-6x+0.024, r=0.9997. The detection limit was 0.8 mg/kg, with the precision of 1.5%-3.6%. The extraction recoveries with the method were 93.5%-101.6%. Conclusion This method is simple, rapid and sensitive. Besides, it is suitable to determine the thiocyanate in milk.