2007•Liquor-making Science & TechnologyRequires access
Determination of Acidic Cellulase Activity
Deng Zhen-kun
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Abstract
Acidic cellulase activity was determined by DNS method.The effects of enzymatic reaction time,pH value,enzyme dilution degree and temperature on the determination results were investigated.The results suggested that the best enzymatic reation conditions were as follows: 30 min enzymatic reaction,pH value as 5.0,enzyme solution diluted to 130 times,and temperature at 50 ℃.Under the above conditions,cellulase activity could reach 1685 IU/g.