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2010•Lishizhen Medicine and Materia Medica ResearchRequires access

Extraction of Alkaloid from Catharanthus roseus by Enzymatic Method

Xiaoxia Yan

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Abstract

Objective To study the effect of cellulose enzyme on extraction of alkaloid from Catharanthus roseus.Methods HPLC method was used to determine the contents of vinblastine,catharanthine and vindoline from C.roseus.Results Compared with the traditional acidic water extraction,the enzymatic method extraction yield of vinblastine,catharanthine and vindoline was increased respectively.Conclusion The yield of vinblastine,catharanthine and vindoline from C.roseus can be effectively increased by using enzymatic extraction process.It is available to use enzyme in extraction alkaloid from C.roseus.

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What this paper is about

Objective To study the effect of cellulose enzyme on extraction of alkaloid from Catharanthus roseus.Methods HPLC method was used to determine the contents of vinblastine,catharanthine and vindoline from C.roseus.Results Compared with the traditional acidic water extraction,the enzymatic method extraction yield of vinblastine,catharanthine and vindoline was increased respectively.Conclusion The yield of vinblastine,catharanthine and vindoline from C.roseus can be effectively increased by using enzymatic extraction process.It is available to use enzyme in extraction alkaloid from C.roseus.

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Available abstract

Objective To study the effect of cellulose enzyme on extraction of alkaloid from Catharanthus roseus.Methods HPLC method was used to determine the contents of vinblastine,catharanthine and vindoline from C.roseus.Results Compared with the traditional acidic water extraction,the enzymatic method extraction yield of vinblastine,catharanthine and vindoline was increased respectively.Conclusion The yield of vinblastine,catharanthine and vindoline from C.roseus can be effectively increased by using enzymatic extraction process.It is available to use enzyme in extraction alkaloid from C.roseus.

Key concepts: Vindoline, Catharanthus roseus, Alkaloid, Vinblastine, Extraction (chemistry), Chromatography, Chemistry, Yield (engineering)

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