2005•Food ScienceRequires access

Isolation and Purification of Lactoferrin in Bovine Colostrums and Delection of Its Immune Function

Weiyi Yu

Abstract

The lactoferrin was purified by centrifugation and ammonium sulfate precipitation from the bovine colostrums. Thepurity of lactoferrin after CM-Sephadex C50 ion-exchange chromatograph and Heparin-Sepharose CL-6B affinity chromato-graph was 76.98%and 92.75% respectively. The two kinds of lactoferrin isomers were assayed by SDS-PAGE and HPLC withthe molecular weights 76000Da and 78000Da respectively. The purified sample and standard lactoferrin showed the sameimmunogens through immunoelectrophoresis. The isolated lactoferrin improved the immune function significantly in miceexperiments.

About this research paper

What this paper is about

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Lactoferrin, Chromatography, Chemistry, Sephadex, Ammonium sulfate precipitation, Centrifugation, Immunoelectrophoresis, Size-exclusion chromatography