Experimental Study on the Impact of Chinese Medicine Shang Xiao Decoction to the Epithelial Cell Apoptosis of Radiation Esophageal Injury of Rats

Objective:To observe the effect of shang Xiao decoction(SXD) to the epithelial cell apoptosis of radiation esophageal injury of rats and to investigate the mechanism.Methods:One hundred SD male rats were randomly divided into 5 groups:blank control group,model group,saline group,Amifostine group and SXD group.The upper paragraph esophaguses of all rats except those in the blank group were given single dosese of radiation of 43Gy by 6MV-X ray.10 rats in each group were randomly killed humanely on the 7th and 14th day after irradiation.Then the esophaguses were removed and sectioned.The esophageal epithelial cell apoptosis,Bcl-2 and bax gene expression and TNF-α levels of Peripheral blood were detected.Results:on the 7th and 14th day after irradiation,the cell apoptosis in esophageal epithelial tissue was markedly increased.Compared with the model group,the epithelial apoptotic cell was reduced significantly in esophageal mucosa of the SXD group(P 0.01) .The Bcl-2 protein expression and Bcl-2 /Bax ratio were also higher Significantly(P 0.05) and the TNF-α levels of Peripheral blood was lower Obviously in the SXD group than in the model group(P 0.05) .Conclusion:Traditional chinese medicine shang xiao decoction can reduce the epithelial cell apoptosis of radiation esophageal injury and its mechanism may be related to the inhibition of the expression of TNF-α,up-regulating of Bcl-2 expression.