The research on the relationship between the expression of Survivivn and apoptosis at the early stage of rats spinal cord injury

Objective:To study the relationship between Survivin expression of rat spinal cord tissue and cell apoptosis during different periods of spinal cord injury.Methods:56 healthy adult Wistar rats were randomly divided into fake injury group(controlled group,n = 8) and injury group(experimental group,n = 48); fake injury group only open vertebral boards of T10 and part of T9,and T11,experimental group used improved Allen's methods to establish T10 spinal cord injury model,and then randomly executed 8 rats at different time(12h,and 24h,and 3d,and 5d,and 7d,and 10d) after hurt as experimental materials.We used HE dyeing to observe pathological changes,and used Immunohistochemical method to observe the expression of Survivin in spinal cord tissue.We made a qualitative and quantitative analysis for it and determined cell apoptosis index by means of deoxyribose transferase mediated Nick end labeling(TUNEL method),and conducted a correlation analysis.Results:Rats in control group occasionally have survivin protein expression in rat spinal cord or around ash.In experimental groups,rats have very little survivin protein expression within 12h,but increased at 3d,but then began to decline.There still existed statistical significance at 10d when compared with the control group(P 0.05).In control group,there are a small number of cells apoptosis after spinal cord injury; and in experimental group,there was a large number of apoptotic cells at 12h,3d up to peak.The number was markedly declined at 5d and 7d.At 10 d there still exist small amounts of expression.The difference of cells apoptosis at 24h,3d,7d when compared with those in experimental group are statistically significant(P 0.05).Conclusion:Survivin expression after spinal cord injuries are on the rise and are positively associated with cell apoptosis.Survivin expression after spinal cord injury involved in the inhibition of nerve cell apoptosis and thus to mitigate the effects of spinal cord injury.