Effects of 17β-Estradiol-Enriched Cardiopiegic Solution on Myocardial Ischemic-Reperfusion Injury

Objective: Our aims were to study the effects of 17 β estradiol-enriched cardiopiegic solution on myo-cardial ischemia reperfusion injury (MIRI) and to investigate its mechanism of protective effects in rats. Methods: Thirty rats underwent bilateral ovariectomy were randomly divided into 3 groups:ischemia-reperfusion control (Group I-R) ,dimethyl sulfoxide( DMSO) solvent control (Group D), and 17-estradiol experimental group (Croup E). An isolated heart model of rat was established. Cardiopiegic solution was perfused through aorta. The creatin phosphokinasc ( CPK) , lactec dehydrogenase(LDH) , NO in the coronory effluent, myocardial NOS activity, and the content of myocardial MDA were measured at the 30th minute of reperfusion. We also observed the cardiologic function. Results: After 30-minute reperfusion, the cardiologic function of rats in Croup E recovered well coronory flow and NO production increased, and levels of CPK,LDH, and MDA decreased compared with group I-R and group D. The myocardial NOS activity was much higher in group E than that in group I-R and group D. Conclusion: The 17β-estrodiol can increase NOS activity , promote NO production, and protect isolated rat hearts against ischemia-reperfusion injury.