Effects of IKK16 on proliferation and apoptosis of esophageal carcinoma cell line Eca-109

OBJECTIVE To investigate the effects of IκB kinase inhibitor IKK16 on proliferation and apoptosis of esophageal carcinoma cell line Eca-109.METHODS The human esophageal cancer cell line Eca109 was cultured in vitro and divided into three groups:blank control group(no drug added),low concentration group(70 nmol·L-1)and high concentration group(200nmol·L-1).Methyl thiazolyl tetrazolium(MTT)method was used to detect the proliferation of Eca109 cells with drug added after 24,48 and 72 hours.Western blot was used to investigate the expression of p65,IκB,caspase-3 and Bcl-2 48 hours later.RESULTS Compared to blank control group,IKK16 in groups of two concentrations could significantly suppress the proliferation of Eca-109 cells,inhibit expression of p65 in nucleus(P0.05),and increase IκB in cytoplasm(P0.05).IKK16 could also obviously increase expression of caspase-3(P0.05)and reduce expression of Bcl-2(P0.05)in a dose dependent manner.CONCLUSION IKK16 can inhibit proliferation and promote apoptosis of Eca109 cells,therefore,it may be considered as apotential therapeutic drug against esophageal carcinoma.