2012•Suzhou Daxue xuebao. Faxue banRequires access
Full-length cDNA sequence analysis on IRM-2 mouse cDNA library
Feiyue Fan
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Abstract
Objective To analyse cDNA sequences from full-length cDNA library of IRM-2 mice.Methods Full-length cDNA products were amplified by PCR from cDNA library of IRM-2 mice according to twenty-one pieces of EST.Full-length cDNA sequences and properties were analyzed by comparing with GenBank database.Results Five pieces of full-length cDNA were obtained according to PCR products amplified by twenty-one pairs of primers,which were not the same as that of known mice genes.Amino acid sequences and properties of proteins encoded by five pieces of full-length cDNA were acquired.Conclusion Five pieces of full-length cDNA imply that radioresistance correlative genes may be existed in IRM-2 mice,laying a foundation for isolating and identifying radioresistance correlative genes in further study.