RP-HPLC simultaneous determination of chlorogenic acid,caffeic acid and 4-hydroxyacetophenone in herb of Artemisia capillaris Thunb. under different UV-vis wavelengths

Objective:To establish a method for the determination of chlorogenic acid,caffeic acid and 4-hydroxyacetophenone in herb of Artemisia capillaris Thunb.simultaneously under different UV wavelengths.Methods:An RP-HPLC method detected by different UV wavelengths—325 nm for chlorogenic acid and caffeic acid,275 nm for 4-hydroxyacetophenone respectively had been developed:The system consisting of a C18 column (250 mm × 4.6 mm,5 μm ) and a mixture of acetonitrile and 0.04% phosphate acid as the mobile phase was adopted;The flow rate was 1.0 mL·min-1.Results:The linear response range was 52.10-1.303×103 μg·mL-1(r=0.9999),1.756-43.91 μg·mL-1 (r=0.9999) and 0.6574-16.43 μg·mL-1 (r=0.9995),respectively (n=5).The average recoveries (n=9) of chlorogenic acid,caffeic acid and 4-hydroxyacetophenone were 98.1%,98.6% and 100.8%,respectively.Conclusion:The assay demonstrates that the method has adequate accuracy and selectivity to measure the four chemical constituents in herb of Artemisia capillaris.