2008•China Medical HeraldRequires access

Determination of Chlorogenic acid in different flowering periods of Artemisia capillaris Thunb.

Yufeng Fan

Abstract

Objective:To establish a method for the determination of Chlorogenic acid in different flowering periods of Artemisia capillaris Thunb.. Methods:A Thermo SCIENIFIC C18 was used with acetonitrile and 0.4% phosphoric acid solution(13∶87)as mobile phase.The detection wavelength was 327 nm. Results:The calibration curve of the Chlorogenic acid was linear in the range of 9.76-48.80 μg/ml(r=0.999 9).The average recovery was 98.1%,RSD=1.48%. Conclusion:The method is accurate,reliable and reproducible.It can be used for the determination of Chlorogenic acid in different flowering periods of Artemisia capillaris Thunb..

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Key concepts: Chlorogenic acid, Phosphoric acid, Medicine, Artemisia, Calibration curve, Chromatography, Traditional medicine, Linear relationship