Effect of Bacillus licheniformis on Growth Performance,Digestive Enzymes and the Number of Intestinal Main Microbial Flora of Piglets Raised in Fermentation Bed

This study investigated the effect of Bacillus licheniformis on growth performance,digestive enzymes activity of pancreas,disaccharidase of intestinal mucosa and the number of intestinal main microbial flora of weaned piglets raised in fermentation bed.One hundred and eight 35-day-old weaned piglets with similar average body weight were randomly divided into three groups with three replicates in each group and twelve piglets in each replicate.The weaned piglets in three groups were fed a basal diet(control group,CT),basal diet+40 mg/kg bacitracin zinc+20mg/kg colistin sulphate(antibiotic group,ABT)and basal diet+300mg/kg Bacillus licheniformis(probiotics group,PBT).The pre-test period lasted for 7days and the trial period lasted for 52 days.The results showed as follows:1Compared with the control group,the supplementation of Bacillus licheniformis could increase ADG,ADFI and decrease F/G to some extent,but there were no significant differences in growth performance among all groups(P0.05).2Compared with the control group,the supplementation of Bacillus licheniformis extremely signifi-cantly increased the activitiy of amylase in pancreas,the activity of maltase in duodenum mucosa and the activity of lactase in jejunum mucosa(P0.01),and significantly increased the activity of sucrase in duodenum and jejunum mucosa(P0.05).3Compared with the control group,the supplementation of Bacillus licheniformis significantly increased the number of Bacillus in caecum(P0.05),and the number of Lactobacillus was increased by 4.09%,the number of Escherichia coli was decreased by 4.86%,but the differences were not significant(P0.05).In conclusion,supplementation of 300mg/kg Bacillus licheniformis could improve the activity of digestive enzymes in pancreas and intestinal mucosa of piglets,increase the number of Bacillus in caecum,and improve the growth performance to some extent.