ScholarLens Back to search
2005•Zhongguo yaolixue tongbaoRequires access

Effects of procyanidins on apoptosis of PC12 cells induced by Aβ_(25-35) and its mechanism

Qifeng Zhu

Open publisher page 0 citations

Abstract

Aim To study the effects of Procyanidins (PC) on apoptosis of PC12 cells induced by Aβ 25-35. Methods Cell survival rate was evaluated by MTT assay, and apoptosis was analyzed by Hoechst-PI fluorescence staining. The expressions of mRNA and protein for P53 and Bcl-2 were tested by RT-PCR and Western blot. Results Pretreatment with different concentrations of PC (10~30 mg·L -1) for 1h increased the survival rate of PC12 cells in a dose-dependent fashion. PC prevented the PC12 cells nuclei from shrinkage, condensation and cleavage induced by Aβ 25-35. PC decreased the expressions of P53 mRNA and P53 protein, and increased the expression of bcl-2 mRNA and Bcl-2 protein. Conclusion These results indicated that PC can protect PC12 cells from apoptosis induced by Aβ 25-35. The mechanism of protection is likely related to decreasing P53 gene expression, and increasing bcl-2 gene expression.

About this research paper

What this paper is about

Aim To study the effects of Procyanidins (PC) on apoptosis of PC12 cells induced by Aβ 25-35. Methods Cell survival rate was evaluated by MTT assay, and apoptosis was analyzed by Hoechst-PI fluorescence staining. The expressions of mRNA and protein for P53 and Bcl-2 were tested by RT-PCR and Western blot. Results Pretreatment with different concentrations of PC (10~30 mg·L -1) for 1h increased the survival rate of PC12 cells in a dose-dependent fashion. PC prevented the PC12 cells nuclei from shrinkage, condensation and cleavage induced by Aβ 25-35. PC decreased the expressions of P53 mRNA and P53 protein, and increased the expression of bcl-2 mRNA and Bcl-2 protein. Conclusion These results indicated that PC can protect PC12 cells from apoptosis induced by Aβ 25-35. The mechanism of protection is likely related to decreasing P53 gene expression, and increasing bcl-2 gene expression.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Aim To study the effects of Procyanidins (PC) on apoptosis of PC12 cells induced by Aβ 25-35. Methods Cell survival rate was evaluated by MTT assay, and apoptosis was analyzed by Hoechst-PI fluorescence staining. The expressions of mRNA and protein for P53 and Bcl-2 were tested by RT-PCR and Western blot. Results Pretreatment with different concentrations of PC (10~30 mg·L -1) for 1h increased the survival rate of PC12 cells in a dose-dependent fashion. PC prevented the PC12 cells nuclei from shrinkage, condensation and cleavage induced by Aβ 25-35. PC decreased the expressions of P53 mRNA and P53 protein, and increased the expression of bcl-2 mRNA and Bcl-2 protein. Conclusion These results indicated that PC can protect PC12 cells from apoptosis induced by Aβ 25-35. The mechanism of protection is likely related to decreasing P53 gene expression, and increasing bcl-2 gene expression.

Key concepts: Apoptosis, Western blot, Messenger RNA, Molecular biology, Chemistry, MTT assay, Pi, Gene expression

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Effects of procyanidins on apoptosis of PC12 cells induced by Aβ_(25-35) and its mechanism — Research Paper | ScholarLens