Protection effects of hydrogen-rich saline for hepatic ischemia-reperfusion injury by reducing oxidative stress in rats

Objective To investigate the protective effects of hydrogen-rich saline for hepatic ischemia-reperfusion injury by inhibiting the oxidative stress in rats.Methods Thirty two male Wistar rats were randomly allocated into 4 groups:sham operation group,ischemia-reperfusion(IR) group,IR+normal saline(IR+NS) group and IR+hydrogen-rich saline(IR+HS)group.A rat model of hepatic ischemia-reperfusion injury was established in this experiment.After operation for 12 hours,the rats were sacrificed and the serum and liver samples were collected.The AIR and AST levels in serum were determined,liver histological damage was evaluated,and the superoxide dismutase(SOD),Malondialdehyde(MDA)and glutathione(GSH) were also determined.Results The pancreas tissue in IR+HS group was more slightly damaged than those in IR+NS group after operation.The AIR,AST level in IR+HS group were significantly lower than those of the IR+NS group(P0.05).The levels of plasma MDA were lower in IR+HS group than those in IR+NS group(P0.05).The levels of plasma SOD and GSH were higher in IR+HS group than those in IR+NS group(P0.05).Conclusion Hydrogen-rich saline may reduce the damage of hepatic ischemia-reperfusion injury by inhibiting oxidant stress in rats.