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2013•Chinese journal of integrated traditional and Western medicineRequires access

Effects of berberine on the expression of vascular endothelial growth factor in HepG2 cells

Tao Xiuliang

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Abstract

[Objective]To study the effect of berberine on cell proliferation and the expression of vascular endothelial growth factor(VEGF)in HepG2 cells.[Methods]After being treated with different concentrations of berberine for 24h,HepG2 cells growth inhibition was assessed by MTT assay.VEGF levels of mRNA and protein in HepG2 were detected by Real-time PCR and Western blot respectively.[Results]In certain concentration range,berberine inhibited cellular proliferation and down-regulated the mRNA and protein expression of VEGF in dose-dependant manner.[Conclusion]Berberine can inhibit the proliferation of HepG2 cells and expression of VEGF,and anti-angiogenesis may be one of the mechanisms of its antitumor action.

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What this paper is about

[Objective]To study the effect of berberine on cell proliferation and the expression of vascular endothelial growth factor(VEGF)in HepG2 cells.[Methods]After being treated with different concentrations of berberine for 24h,HepG2 cells growth inhibition was assessed by MTT assay.VEGF levels of mRNA and protein in HepG2 were detected by Real-time PCR and Western blot respectively.[Results]In certain concentration range,berberine inhibited cellular proliferation and down-regulated the mRNA and protein expression of VEGF in dose-dependant manner.[Conclusion]Berberine can inhibit the proliferation of HepG2 cells and expression of VEGF,and anti-angiogenesis may be one of the mechanisms of its antitumor action.

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Available abstract

[Objective]To study the effect of berberine on cell proliferation and the expression of vascular endothelial growth factor(VEGF)in HepG2 cells.[Methods]After being treated with different concentrations of berberine for 24h,HepG2 cells growth inhibition was assessed by MTT assay.VEGF levels of mRNA and protein in HepG2 were detected by Real-time PCR and Western blot respectively.[Results]In certain concentration range,berberine inhibited cellular proliferation and down-regulated the mRNA and protein expression of VEGF in dose-dependant manner.[Conclusion]Berberine can inhibit the proliferation of HepG2 cells and expression of VEGF,and anti-angiogenesis may be one of the mechanisms of its antitumor action.

Key concepts: Berberine, Vascular endothelial growth factor, Angiogenesis, Western blot, MTT assay, Messenger RNA, Cell growth, Protein expression

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