2011•Zhongguo mianyixue zazhiRequires access
Explore the effect and mechanism of phloretin on γδT cells kill gastric cancer SGC-7901 cells
Chen Fu
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Abstract
Objective:To explore the effect and mechanism of phloretin on γδT cells kill human gastric cancer SGC-7901 cells.Methods:γδT cells were amplified from human peripheral blood cells through IPP method.After cultured with different concentrations of phloretin for 48 hours,MTT method was used to test the growth curve of γδT cells and SGC-7901 cells;FCM was used to test the expression of PFP and GraB of γδT cells;LDH release method was used to test the cell-killing activity of γδT cells to SGC-7901 cells;Western blot to test the Wnt3a expression of γδT cells.Results:After cultured with IPP for 10 days,γδT cells increased from 3.12% to 79.6%.Compared to the control group,when the concentration of phloretin increased from 2.35 μg/ml to 18.75 μg/ml it could proliferate the γδT cell growth significantly(P0.05)and phloretin at concentration 75 μg/ml could inhibit the gastric cancer SGC-7901 cell growth significantly(P0.05).When the concentration increased from 2.35 μg/ml to 75 μg/ml the killing activity of γδT cells to the SGC-7901 cells enhanced significantly(P0.05);the expression of PFP,GraB and Wnt-3a increased significantly compared to the control group(P0.05).Conclusion:Phloretin can enhance the killing effect of γδT cells to SGC-7901 cells,the mechanism may be related to the phloretin could proliferate the γδT cell growth,increase the expression of PFP,GraB and activate the Wnt signaling pathway.