Research of Sulindac effect on human’s γδT cells

Objective:To research the relationship of Sulindac and human’s γδT cells on preventing digestive tract’s tumor. Methods:Use the isopentenyl pyrophosphate method to amplify human peripheral blood γδT cells in vitro. After different concentration’s Sulindac to induce γδT and SGC-7901 cells, flow cytometry was used to detect the cell surface marker of γδT cells before and after cultivation and the apoptosis percentage of γδT cells and SGC-7901 by Sulindac. Meanwhile, the killing and wounding activity of γδT cells was measured by lactate dehydrogenase method. Results:γδT cells were amplified from 4.21%to 70.35% in 10 days, CD44 up to 94.0%. when Sulindac’s density is 100 μmol/L, the growth inhibition ratio of γδT cells that up to 42.1% was notably higher than gastric carcinoma cell SGC-7901’s growth inhibition ratio(7.4%). After γδT cells and SGC-7901 induced by different concentration of Sulindac for 24 h, the cytotoxicity to SGC-7901 is the highest under 12.5 μmol/L Sulindac. The apoptosis percentage of γδT cells(52.71%) was significantly higher than SGC-7901’s apoptosis percentage(7.88%) after induced by Sulindac(100 μmol/L) for 24 h. Conclusion:Sulindac can increase the effect of γδT cells killing tumor cells in clinical routine drug concentration, while it can strikingly inhibit the reproductive and killing activity of γδT cells if its concentration higher than routine dose, but this inhibition effect is not obvious for gastric carcinoma cell. This result may offer an experimental base for applying clinical NSAIDs to prevent digestive tract’s tumor.