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2008•Journal of Huaiyin Institute of TechnologyRequires access

Studies on Determination Method of Ginkgolic Acids by RP-HPLC

Hongfeng Yan

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Abstract

This paper,through examining wave length,pH value of mobile phase,the mobile phase composition and column temperature,etc.,has established the RP-HPLC determination method of ginkgolic acids.The sample can be directly analyzed by HPLC after the pretreatment.The recovery is 96.50%-101.67%,RSD(n=5) is 1.50%;linear range is from 0.518μg to 9.897μg(correlation coefficient r=0.9998),and the lowest examination limits is 6.7ng.The method is simple and accurate and has wider linear range.

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What this paper is about

This paper,through examining wave length,pH value of mobile phase,the mobile phase composition and column temperature,etc.,has established the RP-HPLC determination method of ginkgolic acids.The sample can be directly analyzed by HPLC after the pretreatment.The recovery is 96.50%-101.67%,RSD(n=5) is 1.50%;linear range is from 0.518μg to 9.897μg(correlation coefficient r=0.9998),and the lowest examination limits is 6.7ng.The method is simple and accurate and has wider linear range.

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Available abstract

This paper,through examining wave length,pH value of mobile phase,the mobile phase composition and column temperature,etc.,has established the RP-HPLC determination method of ginkgolic acids.The sample can be directly analyzed by HPLC after the pretreatment.The recovery is 96.50%-101.67%,RSD(n=5) is 1.50%;linear range is from 0.518μg to 9.897μg(correlation coefficient r=0.9998),and the lowest examination limits is 6.7ng.The method is simple and accurate and has wider linear range.

Key concepts: Chromatography, High-performance liquid chromatography, Correlation coefficient, Linear correlation, Linear relationship, Linear range, Phase (matter), Analytical Chemistry (journal)

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