Effect of NEP1-40 in inhibiting neurite outgrowth on neonatal rats with hypoxic ischemic brain damage

【Objective】 To explore the effect of Nogo-A receptor antagonist in inhibiting neurite outgrowth in neonatal rats with hypoxic ischemic braindamage(HIBD). 【Methods】 100 Wistar rats of 7 days were randomly divided into 10 sets at 2 different times(6 h,24 h):the sham-operation set and the normal control set;the hypoxie-ischemic brain damage(HIBD)set and HIBD after treated with NEP1-40 set(NEP1-40 set)and HIBD after treated with ganglioside set(GM set) by immunohistochemistry SP method to observe the expression of positive cells. 【Results】 The expression of positive cells in the two times HIBD sets was obviously higher than the corresponding sham-operation set;NEP1-40 could inhibit the expression of Nogo-A in HIBD rats,NEP1-40 set had obviously statistical difference with HIBD set;The expresson of Nogo-A in GM set at HI 6 h had no difference with HIBD set but lower than HIBD set at 24 h and higher than NEP1-40 set(P0.05). 【Conclusion】 The expression of Nogo-A goes up after hypoxic-ischemic brain damage,it may inhibit neurite outgrowth of the central nervous system of HIBD,NEP1-40 can antagonize this effect,and promote neurite outgrowth in HIBD.