Expressions of CD4~+CD_(45)RA and CD4~+CD_(45)RO in Peripheral Blood CD4~+T Lymphocytes in Children with Bronchial Asthma

Objective To explore the expressions of CD4+CD45RA and CD4+CD45RO in children with bronchial asthma and its clinical significance.Methods There were 28 asthma attacking patients,27 asthma remission patients and 20 healthy children.The percentage of CD4+CD45RA and CD4+CD45RO in T lymphocytes in peripheral blood were determined using fluorescein isothiocyanate(FITC) labelled an-ti-CD4 monoclonal antibody,Phycoerythrin(PE) labelled anti-CD45RA monoclonal antibody and PE-cyanine 5(PE-Cy5) Labelled anti-CD45RO monoclonal antibody by flow cytometry.SPSS 13.0 software was used to analyze all data.Results Patients with asthma attacking had a significantly higher percentage of CD4+CD45RO+ than that of other 2 groups(q=9.50,8.30 Pa0.05).Significantly lower percentage of CD4+CD45RA+ and lower ratio of CD4+CD45RA+/CD4+CD45RO+ were found in patients with asthma attacking than those in other 2 groups(q=12.47,8.39 Pa0.05;q=8.96,6.21 Pa0.05).The percentage of CD4+CD45RA+ in asthma remission patients was significantly higher than that in control group(q=3.08 P0.05).However the percentage of CD4+CD45RO+ and the ratio of CD4+CD45RA+/CD4+CD45RO+ in asthma remission patients were not markedly different from the control subjects(q=0.45,2.02 Pa0.05).Conclusion The dysequilibrium of CD4+CD45RA+and CD4+CD45RO+ on T lymphocytes in peripheral blood may be one pathogenesis of bronchial asthma.