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Folin-Ciocalteu Colorimetric Determination of Total Polyphenols Content in Walnut Kernel

LI Shu-fan

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Abstract

With 70 % methanol ultrasonic extraction of total polyphenols in walnut kernel,Folin-Ciocalteu colorimetry method to determine total polyphenols in Walnut kernel was developed. The results showed that the reaction was carried out with 0. 5 mL of Folin-Ciocalteu reagent and 8mL of 10 % aqueous Na2CO3solution in temperature 25 ℃ and reaction time 2. 5h. The detection wavelength was at 770 nm. Total polyphenols content and absorbency is good linear relationship. The average spiked recovery was 98. 4 %,with a RSD of 4. 28 %. This method is easy to operate with fast,sensitive,and stable determination of total polyphenols in mulberry fruits.

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What this paper is about

With 70 % methanol ultrasonic extraction of total polyphenols in walnut kernel,Folin-Ciocalteu colorimetry method to determine total polyphenols in Walnut kernel was developed. The results showed that the reaction was carried out with 0. 5 mL of Folin-Ciocalteu reagent and 8mL of 10 % aqueous Na2CO3solution in temperature 25 ℃ and reaction time 2. 5h. The detection wavelength was at 770 nm. Total polyphenols content and absorbency is good linear relationship. The average spiked recovery was 98. 4 %,with a RSD of 4. 28 %. This method is easy to operate with fast,sensitive,and stable determination of total polyphenols in mulberry fruits.

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Available abstract

With 70 % methanol ultrasonic extraction of total polyphenols in walnut kernel,Folin-Ciocalteu colorimetry method to determine total polyphenols in Walnut kernel was developed. The results showed that the reaction was carried out with 0. 5 mL of Folin-Ciocalteu reagent and 8mL of 10 % aqueous Na2CO3solution in temperature 25 ℃ and reaction time 2. 5h. The detection wavelength was at 770 nm. Total polyphenols content and absorbency is good linear relationship. The average spiked recovery was 98. 4 %,with a RSD of 4. 28 %. This method is easy to operate with fast,sensitive,and stable determination of total polyphenols in mulberry fruits.

Key concepts: Polyphenol, Colorimetry, Chemistry, Chromatography, Reagent, Extraction (chemistry), Methanol, Content determination

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