Immune Regulation of Shufeng Liangxue Decoction on Immune Thrombocytopenic Purpura Model in Mice

Objective:To observe the impact influence of Shufeng Liangxue decoction of on platelet count,interferon-γ(IFN-γ),CD80,CD86 and programmed cell death gene(PD-1) of the model mousein of peripheral blood in disease of immune thrombocytopenic purpura(ITP) model mice in peripheral blood on treatment with Shufeng Liangxue decoction.Method:It was designed to use guinea pig anti-mouse guinea pig platelet serum(APS) was injected into mice continuously in order to induce the immune thrombocytopenic purpura mouse models.Then mice of Shufeng Liangxue treatment groups,positive group,model group and control group were administered orally with Shufeng Liangxue decoction(112.5,75,37.5 g·kg-1·d-1)of large,medium and small doses prednisone(10 mg·kg-1·d-1)and saline respectively,and all groups were maintained its treatment for continuel 7 days.The blood cell counter was used to detect platelet count,The the levels of cytokine IFN-γ was detected by enzyme linked immunosorbentassay(ELISA),CD80,CD86 and PD-1 were detected by flow cytometry.Result:Platelet count reduced and IFN-γ,TCD80,CD86 and PD-1 increased significantly in model group mice.Platelet count increased significantly(P0.05)and IFN-γ,TCD80,CD86 and PD-1 significantly decreased(P0.05) in Shufeng Liangxue decoction group mice.Conclusion:The Shufeng Liangxue decoction could enhance platelet count decrease IFN-γ,CD80,CD86 and PD-1express,improve unbalance of cellular immunity function in ITP model miceto to treat ITP.